Advanced Protein Characterization Services
At Eagle Analytical, we provide cutting-edge LC-MS–based protein characterization to support the development, analysis, and optimization of biotherapeutics. Our team of expert scientists leverage deep analytical expertise and state-of-the-art instrumentation to deliver high-quality, reliable data paired with meaningful scientific interpretation.
From sequence confirmation to post-translational modification analysis, our comprehensive protein characterization services empower clients to confidently advance their programs and make informed decisions at every stage of development.
Peptide Mapping and Sequence Confirmation
Peptide mapping is a widely used analytical method for confirming a protein amino acid sequence and identifying potential structural changes. The technique combines enzymatic digestion with liquid chromatography and mass spectrometry to generate and analyze peptide fragments.
The process uses specific proteolytic enzymes to digest protein into smaller peptides.
These peptide fragments are then separated using liquid chromatography (LC) and analyzed using high-resolution mass spectrometry (MS).
GLP-1 Peptide High-Throughput Characterization
GLP-1–based therapeutics and peptide analogs require robust analytical characterization to ensure sequence integrity, purity, and product consistency. Eagle provides high-throughput LC-MS characterization workflows designed to rapidly analyze GLP-1 peptides across large sample sets.
Our approach enables accurate confirmation of peptide identity, detection of sequence variants and impurities, and consistent evaluation of peptide quality to support discovery, development, and manufacturing quality.
Protein Identification and Quantitation
Accurate protein identification and quantitation are critical for confirming sample composition, evaluating protein expression, and supporting regulatory submissions. Eagle uses advanced LC-MS–based workflows to provide confident protein identification and precise quantitative measurements across a wide range of biological samples.
These analyses support applications such as protein expression studies, comparative protein analysis, and characterization required for biologic development and regulatory documentation.
Intact Protein Analysis
(mAbs, ADCs, and Complex Biologics)
Intact protein analysis provides critical insight into the molecular integrity and heterogeneity of biologic therapeutics.
Using high-resolution mass spectrometry, Eagle characterizes intact proteins—including monoclonal antibodies (mAbs), antibody-drug conjugates (ADCs), and other complex biologics—to confirm molecular weight, evaluate structural variants, and assess drug-to-antibody ratios.
These analyses support comprehensive characterization required for development, comparability studies, and regulatory submissions.
Post-Translational Modification (PTM) Analysis
Post-translational modifications (PTMs) play a key role in protein structure, stability, and biological activity.
Eagle provides detailed LC-MS analysis to identify and characterize critical PTMs, including oxidation, deamidation, glycosylation, phosphorylation, and other modifications that can impact product quality and function.
Frequently Asked Questions about Protein Characterization
Protein characterization is the process of analyzing proteins to understand their structure, sequence, and modifications. Eagle provides advanced LC-MS–based protein characterization, helping biopharmaceutical companies ensure the quality, safety, and efficacy of biologics, including monoclonal antibodies and peptide therapeutics.
High-throughput GLP-1 peptide characterization allows rapid, accurate analysis of peptide identity, purity, and sequence integrity across large sample sets.
This accelerates development timelines while ensuring consistent product quality for GLP-1 therapeutics and peptide-based drugs.
Intact protein analysis evaluates molecular weight, structural integrity, and heterogeneity of biologics such as monoclonal antibodies (mAbs) and antibody-drug conjugates (ADCs).
These insights are essential for comparability studies, quality assessment, and advanced protein characterization strategies.
Eagle uses high-resolution LC-MS to perform peptide mapping and sequence confirmation, verifying protein sequences and detecting any variations or impurities.
Protein identification and quantitation are critical for confirming sample composition and measuring protein expression levels.
LC-MS–based methods deliver precise, reproducible results that support biologic research, process development, and regulatory documentation.
PTM analysis identifies critical modifications such as glycosylation, oxidation, and phosphorylation that influence protein stability and function.
Comprehensive LC-MS–based PTM characterization ensures deeper understanding of biologic performance and supports robust quality control.
Get Started Today
Looking for Protein Analysis Solutions?
Call the Eagle Client Care Team at (800) 745-8916 to discuss your operation’s specific needs and answer any questions!